Characterization, germination capacity and different pre-sowing on seed germination of Diospyros crassiflora Hiern.

The jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of its seeds and the problem of viability these seeds. The present study aims was to make a summary study of the fruits and seeds and to evaluate the different parameters of germination, seed conservation of D. crassiflora. The fruit is washed by hand with tap water. For the morphological study of the fruits, 47 fruits were randomly selected from all the fruits collected. The following parameters were recorded: length, diameter, fresh weight and total number of manually extracted seeds per fruit. Subsequently, seeds removed by dissection of the fruits were cleaned to remove the different tissues (mesocarp, endocarp) surrounding the seeds. For seed characterisation, a sample of 105 fresh seeds was taken for size and weight measurements. Seeds that appeared healthy were randomly selected for germination experiments. Periodic germination tests were carried out to test the viability and germination capacity of the seeds as a function of post-harvest shelf life. Seeds were tested immediately after harvest and then on the following days (4, 7, 14, 21, 28, 35, 42 and 49) for 2 months with six replicates of 10 seeds per treatment. The germination rate, duration and germination time were calculated. - The experiment on the characterization of fruits and seeds lasted 2 days for the measurement of the different parameters - The germination test experiment lasted 44 days. - The experiment on the germination capacity of the seeds lasted 56 days.

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Field Value
Creator Tchouga, Alvine Ornella
Creator Affiliation International Institute of Tropical Agriculture (IITA)
Creator ID Type ORCID
Creator ID 0000-0002-5346-068X
Subject Vocab (AGROVOC/GACS/CAB) Germination,Seed treatment,Callogenesis,Tissue culture,Cutting,Somatic embryogenesis
Subject(s) Diospyros crassiflora,Biochemical analysis,Capacity of germination,Pre-sowing
Publisher International Institute of Tropical Agriculture (IITA)
Contributor Person Deblauwe, Vincent; Niemenak, Nicolas; Rachid, Hanna; Forgione, Giovannii
Contributor Role Supervisor
Contributor Project Lead Organisation Center International Institute of Tropical Agriculture (IITA)
Contributor Project Lead Center International Institute of Tropical Agriculture (IITA)
Contributor CRP CGIAR Research Program on Forests,Trees and Agroforestry (FTA)
Contributor Partner The Congo Basin Institute (CBI)
Contributor Donor Mr Bob Taylor
Contributor Project Tissue culture and biochemical analysis of Diospyros crassiflora H. (Ebenaceae): a new approche of propagation and conservation in Cameroon's agroforestry system
Contributor Affiliation International Institute of Tropical Agriculture (IITA)
Open-Access status Limited Access
Production Date 2019-11-01
Embargo End Date 2021-04-24
Content Type Dataset
File Format csv
Identifier Type DOI
Identifier https://doi.org/10.25502/ka20-6m86/d
Identifier Citation Tchouga A.O., Deblauwe V., Forgione G., Hanna R. and Niemenak N. Characterization, germination capacity and different pre-sowing on seed germination of Diospyros crassiflora Hiern.
Source Tissue culture and biochemical analysis of Diospyros crassiflora H. (Ebenaceae): a new approche of propagation and conservation in Cameroon's agroforestry system
Language English
Relation Not applicable
Agroecological Zone Humid warm tropics
Coverage Region Central Africa
Coverage country Cameroon
Coverage Admin Unit Not applicable
Coverage Y (Latitude) 3.84803
Coverage X (Longitude) 11.50208
Coverage Start Date 2017-09-28
Coverage End Date 2019-01-11
Contact Tchouga, Alvine Ornella; International Institute of Tropical Agriculture (IITA)
Contact Email a.tchouga@cgiar.org
Restriction CC-BY 4.0
Email Permission Not applicable
Rights CC-BY 4.0