Variations in microtuber formation among accessions of D. alata and D. rotundata plantlets conserved at IITA's Genetic Resources Center

The conservation of yam genetic resources using field genebanks, in vitro plantlets, pollen and seed storage are constrained by high losses and space requirements, maintenance cost and an irregular flowering, respectively. Microtubers produced from in vitro plantlets are proposed for conservation, as they have a longer shelf-life due to dormancy, and are also hardier and less bulky than plantlets. This study evaluated microtuber formation by in vitro plantlets of 65 and 320 accessions of D. alata and D. rotundata. Plantlets were evaluated for six microtuberization (MTZ) traits using the nested design. Data were subjected to ANOVA and regression at p=0.05 while accessions were clustered using the fastclus procedure of the SAS. Means were separated. Accessions of each species differed for all traits, while species differences were insignificant for MTZ frequencies. High root vigour increased MTZ frequencies in both species, but for D. rotundata, high shoot vigour reduced MTZ frequencies. Out of the 65 accessions of D. alata, 27 (equivalent to 41.54%) and 173 out of 320 D. rotundata accessions (equivalent to 54.06%) had at least 50% tuberization. Half of the accessions of both species had less than 10% tuberization. Microtuber production could be a viable strategy in yam germplasm conservation and propagation especially when dormancy can be adequately controlled.

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Data and Resources

Additional Info

Field Value
Creator Balogun, Monsurat
Creator ID Type ORCID
Creator ID 0000-0001-8770-5529
Subject Vocab Yam,Plantlet,Tuber,Root,Culture,Shoot,Replication,Dioscorea rotundata,Dioscorea alata,Germplasm
Subject(s) Yam
Publisher International Institute of Tropical Agriculture (IITA)
Contributor Person Quat Ng, Iyiola Fawole, Robert Asiedu
Contributor Project Lead Organisation Center International Institute of Tropical Agriculture (IITA)
Contributor Project Lead Center International Institute of Tropical Agriculture (IITA)
Contributor CRP CGIAR Research Program on Roots, Tubers and Banana(RTB)
Contributor Partner Not Applicable
Contributor Donor International Institute of Tropical Agriculture (IITA)
Contributor Project Not Applicable
Contributor Affiliation International Institute of Tropical Agriculture (IITA)
Open-Access status Open Access
Production Date 2002-06-01
Embargo End Date
Content Type Dataset
File Format csv
Identifier Type DOI
Identifier https://doi.org/10.25502/NDBQ-P469
Identifier Citation 1. Balogun, M. O., N.Q.Ng, I. Fawole and H. Kikuno (2007). Variations in microtuberization among local, improved and exotic yam accessions in Nigeria. Proceedings of the 20th Annual Conference of the Biotechnology Society of Nigeria. Ebonyi State University, Abakaliki. 44-47pp. 2. Balogun, M.O. (2005). Development of Microtuber production and dormancy control protocols for yams (Dioscorea spp.) germplasm conservation. Ph.D Thesis, University of Ibadan, Nigeria. 156pp.
Source Root and Tuber Improvement Program
Language English
Relation Not Applicable
Agroecological Zone Semihumid warm tropics
Coverage Region Western Africa
Coverage country Nigeria
Coverage Admin Unit Not Applicable
Coverage X 7.5014
Coverage Y 3.9099
Coverage Start Date 2000-06-01
Coverage End Date 2002-06-01
Contact Balogun, Morufat; Tissue Culture Specialist, Yam Improvement for Income and Food Security in West Africa (YIIFSWA), International Institute of Tropical Agriculture (IITA)
Contact Email m.balogun@cgiar.org
Restriction Not Applicable
Email Permission Not Applicable
Rights CC-BY 4.0