Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern

The jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of its seeds and the long time required for rooting formation of cuttings are limiting its propagation. The present study aims to develop a successful protocol for ebony micropropagation. In vitro culture of nodal segments from seedlings was performed on half-strength Murashige and Skoog medium supplemented with either zeatin (0.0, 2.3, 4.6, 9.1, 13.7, 18.2, 22.8 and 27.4 µM) or 6-benzylaminopurine (BAP) (0.0, 2.2, 4.4, 8.8, 13.3, 17.8, 22.2 and 26.6 µM). After 12 weeks, all media allowed shoot bud-break. Shoots displaying the highest bud-break were observed with 22.8 µM and 22.2 µM of Zeatin and BAP respectively. The shoots were then transferred to a medium supplemented with indole-3-butyric acid (IBA) and phloroglucinol (PG) at different concentration for root induction. Rooted plants were acclimatized to the greenhouse.

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Field Value
Creator Tchouga, Alvine Ornella
Creator Affiliation International Institute of Tropical Agriculture (IITA)
Creator ID Type ORCID
Creator ID 0000-0002-5346-068X
Subject Vocab (AGROVOC/GACS/CAB) In vitro culture,Microcuttings,Plant growth regulators,Callogenesis,Cutting,Tissue,Somatic embryogenesis,Germination
Subject(s) Diospyros crassiflora,African rainforest ebony,Culture plant,Biochemical analysis
Publisher International Institute of Tropical Agriculture (IITA)
Contributor Person Deblauwe,Vincent; Niemenak, Nicolas; Rachid, Hanna; Forgione, Giovannii; Djabou Mouafi, Stephanie Astride
Contributor Role Supervisor
Contributor Project Lead Organisation Center International Institute of Tropical Agriculture (IITA)
Contributor Project Lead Center International Institute of Tropical Agriculture (IITA)
Contributor CRP CGIAR Research Program on Forests,Trees and Agroforestry (FTA)
Contributor Partner The Congo Basin Institute (CBI)
Contributor Donor Mr Bob Taylor
Contributor Project Tissue culture and biochemical analysis of Diospyros crassiflora H. (Ebenaceae): a new approche of propagation and conservation in Cameroon's agroforestry system
Contributor Affiliation International Institute of Tropical Agriculture (IITA), The Congo Basin Institute (CBI)
Open-Access status Open Access
Production Date 2018-08-16
Embargo End Date 2021-04-27
Content Type Dataset
File Format csv
Identifier Type DOI
Identifier https://doi.org/10.25502/6bm0-4x60/d
Identifier Citation Tchouga A.O., Deblauwe V., Djabou S. A., Forgione G., Hanna R. and Niemenak N. Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern
Source Tissue culture and biochemical analysis of Diospyros crassiflora H. (Ebenaceae): a new approche of propagation and conservation in Cameroon's agroforestry system
Language English
Relation Not applicable
Agroecological Zone Humid warm tropics
Coverage Region Central Africa
Coverage country Cameroon
Coverage Admin Unit Not applicable
Coverage Y (Latitude) 3.84803
Coverage X (Longitude) 11.50208
Coverage Start Date 2017-12-23
Coverage End Date 2018-08-16
Contact Tchouga, Alvine Ornella; International Institute of Tropical Agriculture (IITA)
Contact Email a.tchouga@cgiar.org
Restriction CC-BY 4.0
Email Permission Not applicable
Rights CC-BY 4.0