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Standard Operating Procedure (SOP) for Banana Embryo Culture
Banana/plantain breeding depends on successful controlled hybridization (or open pollinations) resulting in the formation of fertile seeds. However, most banana seeds fail to... -
Standard Operating Procedure (SOP) for banana tissue culture media preparation
Plant tissue culture involves growing plant cells, tissues, or organs on a sterile growth medium in vitro under controlled conditions of temperature, light, and humidity. An... -
Standard Operating Procedure (SOP) Site Selection & Field Layout
Site selection and field plot layout are important components of experimentations and require adequate attention. Poor site selections and field layouts are major sources of... -
Guidelines to prepare for digital data collection
This document lists the different tasks to be performed by the use case team before and during the enumerators’ training to ensure digital data collection in the field is smooth. -
Standard Operating Procedure for Yam Data Collection
Accurate data collection is essential for precise decisions in yam breeding activities. It is relevant for reliable choices in selecting and advancing desirable genotypes. -
Standard Operating Procedure for Yam Data Management
The yam breeding generates thousands of genotypic and phenotypic data every year from genetic studies and breeding trials. There is a need for data centralization, curation,... -
Standard Operating Procedures for Genotyping in Yam Breeding Program
Yam (Dioscorea species) leaf samples contain high levels of polysaccharides and polyphenolic compounds. The extraction of DNA from leaves is usually problematic. Therefore,... -
Standard Operating Procedure for Harvesting and Post-Harvest Tuber Management...
The tuber is the food organ and the essential part of the yam plant. Evaluations and selection are premised on this economic part, just like in other root ad tuber crops. The... -
Standard Operating Procedure for Yam Trial Management
Yam trial management involves all the activities implemented throughout the life cycle of a yam trial/experiment, either in the field, screenhouse and laboratory, following a... -
Standard Operating Procedure for Regional Varietal Trial
The yam breeding program executes regional varietal trials (RVTs) in collaboration with its national partners in different countries. The objective of the trial is to... -
Standard Operating Procedure for Yam Multiplication in Semi-Autotrophic Hydro...
Semi-Autotrophic Hydroponics (SAH) is a robust technique for efficiently and rapidly multiplying clonally propagated crops. It is a low-cost and licensed method for high ratio... -
Standard Operating Procedure for Field Trial Labelling and Barcoding
Barcode labels are used for trials plot, genotypes identification and tracking purposes. For the labelling of a trial, this includes stages of advancement, the location of the... -
Standard Operating Procedure for Yam Crossing
Hybridization in yam is a process of transferring of male pollens to female flowers to generate viable seeds with desired genetic recombination of the parents. Hybridization... -
Standard Operating Procedure for Establishment of Yam Seedling Nursery
Seedling nursery involves the raising and management of seedlings from botanical seeds for the purpose of progeny or population development. This is a major stage that succeeds... -
Standard Operating Procedure (SOP) for Trial Design and Establishment
The design of experiments and how it is established have significant impact on the quality of data and the decisions made based on such data thereof. Properly designed... -
Standard Operating Procedure (SOP) for Harvesting
Harvesting is another key activity in the cowpea breeding program that requires much attention. This is the stage where varietal seed mixing can occur. Timely harvesting is... -
Standard Operating Procedure (SOP) for Data Collection
Data capturing is a key component of any trial, given that it drives the decisions in the breeding programs. Without good data, the main purpose for which the trial is... -
Standard Operating Procedure (SOP) for leaf sampling and genotyping
Genotyping is the process of determining differences in the genetic make-up (genotype) of an individual by examining the individual's DNA sequence using biological assays and... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP...
Genotyping involves studying an organism’s genetic makeup based on its DNA. Banana leaves are commonly used for DNA extraction due to their abundance, accessibility, and the... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP...
Genotyping involves studying an organism’s genetic makeup based on its DNA. Banana leaves are commonly used for DNA extraction due to their abundance, accessibility, and the...