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Biochemical analysis of proteins, peroxydasiques activities and polyphenols in different explants during micropropagation of Diospyros crassiflora Hiern

The objective of biochemical analysis was to determine the physiological status of uninodal stems in relation to bud break and axillary bud growth. This analysis will enable a biochemical study to be carried out during the process of callogenesis. The biochemical analyses consisted of the extraction and determination of total soluble proteins, total polyphenols and peroxidases. The biochemical analyses were carried out on vitro plants taken from media suitable for the different in vitro culture techniques (stems, leaves, roots at different stages of growth as well as leaf callus, cotyledons), acclimatised vitro plants (leaves, stems, roots) and nursery vivoplants (leaves, stems and roots). The determination of total soluble protein was carried out according to the method of Bradford, (1976) using Coomassie Blue G250. The measurement of peroxidase activity is carried out in plant material according to the method of Thorpe (1978). The determination of phenolic compounds was carried out according to the method of Marigo et al. (1973). For each extract, three readings were made. - The stems were taken during microcutting in the following months: 1st month; 2nd month; 3rd month. - The leaves were removed during microcutting in the third month. - Calls from leaves and cotyledons were taken during callogenesis in the following months: 1st month; 2nd month; 3rd month; 4th month.

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Additional Info

Field Value
Creator Tchouga, Alvine Ornella
Creator Affiliation International Institute of Tropical Agriculture (IITA)
Creator ID Type ORCID
Creator ID 0000-0002-5346-068X
Subject Vocab (AGROVOC/GACS/CAB) Proteins,Peroiydases,Phenolic compounds,Callogenesis,Somatic embryogenesis,Germination,Cutting
Subject(s) Diospyros crassiflora,Tissue culture plant,Biochemical analysis
Publisher International Institute of Tropical Agriculture (IITA)
Contributor Person Deblauwe,Vincent; Niemenak, Nicolas; Rachid, Hanna; Djabou Mouafi, Stephanie Astride
Contributor Role Supervisor
Contributor Project Lead Organisation Center AfricaRice
Contributor Project Lead Center International Institute of Tropical Agriculture (IITA)
Contributor CRP CGIAR Research Program on Roots, Tubers and Banana(RTB)
Contributor Partner The Congo Basin Institute (CBI)
Contributor Donor Mr Bob Taylor
Contributor Project Tissue culture and biochemical analysis of Diospyros crassiflora H. (Ebenaceae): a new approche of propagation and conservation in Cameroon's agroforestry system
Project ID
Contributor Affiliation International Institute of Tropical Agriculture (IITA), The Congo Basin Institute (CBI)
Open-Access status Open Access
Production Date 2019-12-08
Embargo End Date 2021-04-27
Content Type Dataset
File Format csv
Identifier Type DOI
Identifier https://doi.org/10.25502/pcj2-2179/d
Identifier Citation Tchouga A.O., Deblauwe V., Forgione G., Hanna R. and Niemenak N. Biochemical analysis of proteins, peroxydasiques activities and polyphenols in different explants during micropropagation of Diospyros crassiflora Hiern
Source Tissue culture and biochemical analysis of Diospyros crassiflora H. (Ebenaceae): a new approche of propagation and conservation in Cameroon's agroforestry system
Language English
Relation Not applicable
Agroecological Zone Humid warm tropics
Coverage Region Central Africa
Coverage country Cameroon
Coverage Admin Unit Not applicable
Coverage Y (Latitude) 3.84803
Coverage X (Longitude) 11.50208
Coverage Start Date 2019-08-07
Coverage End Date 2019-12-08
Contact Tchouga, Alvine Ornella; International Institute of Tropical Agriculture (IITA)
Contact Email a.tchouga@cgiar.org
Restriction CC-BY 4.0
Email Permission None
Rights CC-BY 4.0