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Standard Operating Procedure for High throughput Phenotyping using NIRS
The near-infrared spectroscopy is a non-destructive and rapid method for predicting the qualitative and quantitative properties of samples. The NIRS spectral regions, which are... -
Standard Operating Procedure (SOP) FOR DNA EXTRACTION
Deoxyribonucleic acid (DNA) extraction is an important process in molecular plant breeding. DNA extraction involves isolating DNA from the nucleus of a cell. Once purified,... -
STANDARD OPERATING PROCEDURE (SOP) FOR LEAF SAMPLE COLLECTION FOR GENOTYPING
Application of deoxyribonucleic acid (DNA) technology in plant breeding offers an opportunity to enhance and accelerate genetic gain. Molecular markers, which are DNA or gene... -
STANDARD OPERATING PROCEDURE (SOP) FOR HARVESTING AND HARVEST EVALUATION IN C...
In cassava breeding, harvesting is carried out to determine the root yield and assess other important traits. It marks the end of the fieldwork for an experimental cassava... -
STANDARD OPERATING PROCEDURES FOR PHENOTYPING DATA MANAGEMENT IN IITA CASSAVA...
Data management involves data collection, curation, analysis, and storage to ensure accessibility to quality data. The goal of data management is to provide breeders with... -
Standard Operating Procedure (SOP): Weed Control and Management
Weed control and management are well-planned field operations carried out to prevent, remove, or limit weed infestations so the crops can grow to attain high productivity. The... -
Standard Operating Procedure (SOP): Trial Management
Field trials are important in cassava breeding research. Cassava breeders conduct a variety of field trials in the process of developing improved varieties. The results of these... -
STANDARD OPERATING PROCEDURE (SOP) FOR SEED PROCESSING, SEED INVENTORY AND MA...
Cassava is primarily grown via stem cuttings (stakes?), but can also be propagated by seeds. Seed propagation is important to create novel gene combinations and superior cassava... -
STANDARD OPERATING PROCEDURE (SOP) FOR HYBRIDIZATION AND CROSSING BLOCK MANAG...
Genetic variation is the basis of selection in plant breeding. Hybridization is the process of making controlled crosses between selected genotypes to provide genetic variation... -
Standard Operating Procedure (SOP) for Phenotyping Banana for Resistance to t...
Plant-parasitic nematodes (PPNs) such as Pratylenchus spp., Helicotylenchus spp., and Radopholus spp. amongst others are a major constraint to sustainable bananas (Musa spp.)... -
Standard Operating Procedure (SOP) for Pollen Tube Growth and Monitoring (PTGM)
This procedure allows for assessing pollen viability, compatibility, and fertilization efficiency, essential for improving hybridization success and enhancing hybrid recovery... -
Standard Operating Procedure (SOP) for Phenotypic Field data collection and m...
This SOP provides detailed procedures for data collection, analysis, and statistical design for hybrid evaluations in the banana breeding program. It ensures data quality,... -
Standard Operating Procedure (SOP) for Collecting, Preparing, Analyzing, and ...
This SOP provides detailed procedures for collecting, preparing, quantifying, analyzing, and assessing banana pollen grains in a consistent and reproducible manner that ensures... -
Standard Operating Procedure (SOP) for land preparation and planting Banana a...
This Standard Operating Procedure (SOP) provides a comprehensive guide to best practices for land preparation and planting of banana and plantain fields. It aligns with... -
Standard Operating Procedure (SOP) for Harvesting Banana and Plantain
This Standard Operating Procedure (SOP) provides comprehensive, detailed guidelines for banana harvest activities that include the fruit handling, the data collection and data... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP)...
Unlike seed crops, clonally propagated crops are difficult and expensive to conserve. With the rapidly evolving genotyping platforms, it is recommended to conserve leaf samples... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP...
Genotyping involves studying an organism’s genetic makeup based on its DNA. Banana leaves are commonly used for DNA extraction due to their abundance, accessibility, and the... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP...
Genotyping involves studying an organism’s genetic makeup based on its DNA. Banana leaves are commonly used for DNA extraction due to their abundance, accessibility, and the... -
Standard Operating Procedure (SOP) for Banana Embryo Culture
Banana/plantain breeding depends on successful controlled hybridization (or open pollinations) resulting in the formation of fertile seeds. However, most banana seeds fail to... -
Standard Operating Procedure (SOP) for banana tissue culture media preparation
Plant tissue culture involves growing plant cells, tissues, or organs on a sterile growth medium in vitro under controlled conditions of temperature, light, and humidity. An...