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Callogenesis induction on different types of explants of Diospyros crassiflora Hiern

The jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of its seeds and the long time required for rooting formation of cuttings are limiting its propagation. The aim of this research was to establish a reproductible protocol of the in vitro callogenesis induction in D. crassiflora. The young leaves below the apical meristem are taken from seedlings about 3 months old and derived from the germination of D. crassiflora seeds. Similarly, immature flower buds are taken from mature trees. These various explants are washed under a stream of tap water for 30 min and sterilized according to well-defined protocols . Leaf fragments were placed 15 explants per petri dish and repeated 4 times. For the staminodes, we grew 50 explants per petri dish and the experiment was repeated 3 times. For cotyledons, the mature seeds of D. crassiflora, once removed from the fruit, were thoroughly washed with tap water and then sanitized. The extraction of the cotyledons from its housing was done with a scalpel with a size 11 scalpel blade. The cotyledon fragments were placed 10 explants per Petri dish due to 6 repetitions. The experimentation lasted 28 days for each treatment for the leaves and cotyledons finally to observe the calluses. While in staminodes 133 days were necessary to observe callus induction.

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Field Value
Creator Tchouga, Alvine Ornella
Creator Affiliation International Institute of Tropical Agriculture (IITA)
Creator email A.Tchouga@cgiar.org
Creator ID Type ORCID
Creator ID 0000-0002-5346-068X
Subject Vocab (AGROVOC/GACS/CAB) Microcuttings,Plant growth regulators,Callogenesis,Cutting,Tissue,Somatic embryogenesis,Germination
Subject(s) Diospyros crassiflora,African rainforest ebony,Culture plant,Biochemical analysis
Publisher International Institute of Tropical Agriculture (IITA)
Contributor Person 1 Vincent Deblauwe
Contributor Person 1 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 1 email v.deblauwe@cgiar.org
Contributor Person 2 Nicolas Niemenak
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Contributor Person 3 Rachid Hanna
Contributor Person 3 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 3 email r.hanna@cgiar.org
Contributor Person 4 Giovannii Forgione
Contributor Person 4 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 4 email G.Forgione@cgiar.org
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Contributor Project Lead Organisation Center International Institute of Tropical Agriculture (IITA)
Contributor Project Lead Center International Institute of Tropical Agriculture (IITA)
Contributor Initiative/CRP CGIAR Research Program on Forests,Trees and Agroforestry (FTA)
Contributor Partner The Congo Basin Institute (CBI)
Contributor Donor Mr Bob Taylor
Contributor Project Tissue culture and biochemical analysis of Diospyros crassiflora H. (Ebenaceae): a new approche of propagation and conservation in Cameroon's agroforestry system
Project ID
Contributor Affiliation International Institute of Tropical Agriculture (IITA), The Congo Basin Institute (CBI)
Open-Access status Open Access
Production Date 2019-08-21
Embargo End Date 2021-04-27
Content Type Dataset
File Format csv
Identifier Type DOI
Identifier https://doi.org/10.25502/b8v6-zk92/d
Identifier Citation Tchouga A.O., Deblauwe V., Forgione G., Hanna R. and Niemenak N. Callogenesis induction on different types of explants of Diospyros crassiflora Hiern.
Source Tissue culture and biochemical analysis of Diospyros crassiflora H. (Ebenaceae): a new approche of propagation and conservation in Cameroon's agroforestry system
Language English
Relation Not applicable
Agroecological Zone Humid warm tropics
Coverage Region Central Africa
Coverage country Cameroon
Coverage Admin Unit Not applicable
Coverage Y (Latitude) 3.84803
Coverage X (Longitude) 11.50208
Coverage Start Date 2018-05-04
Coverage End Date 2019-08-21
Contact Tchouga, Alvine Ornella; International Institute of Tropical Agriculture (IITA)
Contact Email a.tchouga@cgiar.org
Restriction CC-BY 4.0
Email Permission None
Rights CC-BY 4.0