Effects of genotype, maturity and processing methods on the bioactive components and free scavenging activity of fresh orange maize hybrids

Bioactive compounds in foods are responsible for their biological activities, but biotic and abiotic factors may influence their levels. This study evaluated the impact of three genotypes (designated 4, 5, and 7), maturity stages (20, 27, and 34 days after pollination) and processing methods (hydrothermal and dry-heating) on the bioactive constituents (carotenoids, phytate, tannins, vitamin C) and 2,2-diphenyl-2-picrylhydrazyl radical (DPPH∗ ) scavenging activity of fresh orange maize hybrids. Freshly harvested maize cobs of each genotype were subjected to hydrothermal processing at 100◦C and dry-heating with husks and without husks. Carotenoids (lutein, zeaxanthin, β-cryptoxanthin, α-carotene, and total β-carotene) contents of fresh and processed samples were analyzed using HPLC; other bioactive constituents and DPPH∗ scavenging ability were determined using spectrophotometric methods. Genotype had a significant effect on the levels of carotenoids (p < 0.001) and vitamin C (p < 0.05), while genotype (p < 0.001), and processing methods (p < 0.001) had significant effects on DPPH∗ SC50. Maturity stages, processing methods and their interaction also had significant effects (p < 0.001) on the levels of all the bioactive constituents. A positive moderate to strong correlation was observed between (p < 0.001) α-carotene and the following: lutein (r = 0.57), β-cryptoxanthin (r = 0.69), total β-carotene (r = 0.62). However, the relationship between α-carotene and zeaxanthin was positive but weak (r = 0.39). A positive moderate correlation (p < 0.001) was observed between lutein and the following: β-cryptoxanthin (r = 0.57), total β-carotene (r = 0.58), and zeaxanthin (r = 0.52). A positive strong correlation (p < 0.001) was observed between β-cryptoxanthin and each of total β-carotene (r = 0.92) and zeaxanthin (r = 0.63); total β-carotene and zeaxanthin (r = 0.65); while the association between vitamin C and DPPH∗ SC50 was negative and weak (r = −0.38). Generally, genotype 4 and harvesting at 34 days after pollination had the best combination of bioactive constituents and DPPH∗ scavenging ability.

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Field Value
Creator Alamu, Oladeji
Creator Affiliation International Institute of Tropical Agriculture (IITA)
Creator ID Type ORCID
Creator ID 0000-0001-6263-1359
Subject Vocab (AGROVOC/GACS/CAB) Bioactive components,Free radicals,Maturity,Hybrids,Dehusking,Husks,Food analysis,Food processing,Product development,Genotype,Maize
Subject(s) Orange maize,Processing methods
Publisher International Institute of Tropical Agriculture (IITA)
Contributor Person Bussie Maziya-Dixon, Abebe Menkir, Emmanuel Anyachukwu Irondi, Olorunfemi Olaofe
Contributor Role Project Leader
Contributor Project Lead Organisation Center International Institute of Tropical Agriculture (IITA)
Contributor Project Lead Center International Institute of Tropical Agriculture (IITA)
Contributor CRP CGIAR Research Program on Agriculture for Nutrition and Health (A4NH)
Contributor Partner Not applicable
Contributor Donor International Institute of Tropical Agriculture (IITA), Bill and Melinda Gates Foundation (BMGF), HarvestPlus
Contributor Project Bioactive Composition and Free Radical Scavenging Activity of Fresh Orange Maize Hybrids: Impacts of Genotype, Maturity Stages, and Processing Methods
Project ID OPP1019962
Contributor Affiliation International Institute of Tropical Agriculture (IITA)
Open-Access status Open Access
Production Date 2020-04-16
Embargo End Date
Content Type Dataset
File Format csv
Identifier Type DOI
Identifier https://doi.org/10.25502/b07k-v304/d
Identifier Citation Alamu EO, Maziya-Dixon B, Menkir A, Irondi EA and Olaofe O (2021) Bioactive Composition and Free Radical Scavenging Activity of Fresh Orange Maize Hybrids: Impacts of Genotype, Maturity Stages, and Processing Methods. Front. Nutr. 8:640563. doi: 10.3389/fnut.2021.640563
Source Bioactive Composition and Free Radical Scavenging Activity of Fresh Orange Maize Hybrids: Impacts of Genotype, Maturity Stages, and Processing Methods
Language English
Relation Not applicable
Agroecological Zone Subhumid warm tropics
Coverage Region Western Africa
Coverage country Nigeria
Coverage Admin Unit Not applicable
Coverage Y (Latitude) 9.60004
Coverage X (Longitude) 7.99997
Coverage Start Date 2010-04-04
Coverage End Date 2011-08-28
Contact Alamu, Oladeji; Food Science and Technology, International Institute of Tropical Agriculture (IITA)
Contact Email o.alamu@cgiar.org
Restriction CC-BY 4.0
Email Permission None
Rights CC-BY 4.0