In vitro microtuber production and dormancy as affected by some cultural factors

Scarcity of planting materials, pest and diseases are major constraints to yam production. The tissue culture techniques, meristem culture combined with heat therapy has been used to produce high yielding virus-tested plantlets of root crops, which are later multiplied through micropropagation. This procedure, however has low regedaration and multiplication rates in yam. We investigated effects of different plant growth regulators on rate of growth of yam plantlets when applied at different growth phases. Three concentrations each of uniconazole-p (UP), gibberellic acid (GA3), jasmonic acid(JA) and naphthalene acetic (NAA) acids were applied at single node and primary nodal complex (PNC) formation phases in a split-plot design using two genotypes each of white and water yam. Number of nodes per plantlet was recorded after 16 weeks. Results showed that number of new nodes varied significantly with genotype by growth phase interaction and the main effect of PGRs. After 16 weeks, the control, 1.7 μM UP and 0.03 μM JA showed highest means of 7, 9 and 8 nodes per plantlet respectively compared to 2-3 nodes in the GA treatments. We demonstrated that 4900, 8100 and 6400 plantlets could be obtained in one year using 100 nodes by sub-culturing them four times. Addition of PGRs at PNC doubled the number of nodes per plantlet. However the effect of stage of treatment varied with genotypes. The GA and its inhibitor, UP influenced the multiplication rate of yam.


Data and Resources

Additional Info

Field Value
Creator Balogun, Morufat
Creator Affiliation International Institute of Tropical Agriculture (IITA)
Creator ID Type ORCID
Creator ID 0000-0001-8770-5529
Subject Vocab (AGROVOC/GACS/CAB) Dioscorea,Micropropagation,Uniconazole,Growth regulation,Growth,Dioscorea alata,Dioscorea rotundata,Plantlets,Jasmonic acid,Gibberellic acid,Culture,Tissue culture
Subject(s) Yam,Uniconazole-p,Primary nodal complex
Publisher International Institute of Tropical Agriculture (IITA)
Contributor Person Yong Ng, Iyiola Fawole, Hidehiko Kikuno, Robert Asiedu
Contributor Role Other
Contributor Project Lead Organisation Center International Institute of Tropical Agriculture (IITA)
Contributor Project Lead Center International Institute of Tropical Agriculture (IITA)
Contributor CRP CGIAR Research Program on Roots, Tubers and Banana(RTB)
Contributor Partner Not Applicable
Contributor Donor IITA/Core
Contributor Project Root and Tuber Improvement Program
Project ID
Contributor Affiliation International Institute of Tropical Agriculture (IITA)
Open-Access status Open Access
Production Date 2014-01-05
Embargo End Date 2021-12-31
Content Type Dataset
File Format csv
Identifier Type DOI
Identifier Citation Relative effects of Uniconazole-p and other growth regulators on micropropagation ratio of yam (Dioscorea spp.) plantlets at different growth phases, Balogun, M., Ng, S. Y., Fawole, I.*, Shiwachi, H. and Kikuno, H., in: Journal of Root Crops, volume 40, number 2, pages 5-11, ISSN 0378-2409, 2014.
Source Root and Tuber Improvement Program
Language English
Relation Not applicable
Agroecological Zone Subhumid warm tropics
Coverage Region Western Africa
Coverage country Nigeria
Coverage Admin Unit Not applicable
Coverage Y (Latitude) 7.49708
Coverage X (Longitude) 3.90394
Coverage Start Date
Coverage End Date
Contact Balogun, Morufat, Visiting Scientist - Tissue Culture Specialist YIIFSWA Project, International Institute of Tropical Agriculture (IITA)
Contact Email None
Restriction CC-Zero
Email Permission None
Rights CC-Zero