Standard Operating Procedure (SOP) for Banana Embryo Culture

Banana/plantain breeding depends on successful controlled hybridization (or open pollinations) resulting in the formation of fertile seeds. However, most banana seeds fail to germinate. In vitro embryo culture, which involves the extraction and growth of embryos on suitable culture media for regeneration into plantlets is routinely done in breeding programs to maximize the recovery of progenies from hybridizations.