Standard Operating Procedure (SOP) for banana tissue culture media preparation

Plant tissue culture involves growing plant cells, tissues, or organs on a sterile growth medium in vitro under controlled conditions of temperature, light, and humidity. An ideal plant tissue culture medium should have all the required minerals, nutrients, and vitamins which are sometimes supplemented with growth regulators and solidifying agents. Plant tissue culture is an integral part of banana breeding where it is used for embryo germination and micropropagation through shoot tip culture, requiring Murashige and Skoog |(MS) basal medium supplemented with various growth regulators. Morel and Wetmore vitamins (MWV) is used in place of MS vitamins for embryo germination.