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STANDARD OPERATING PROCEDURE (SOP) FOR LEAF SAMPLE COLLECTION FOR GENOTYPING

Application of deoxyribonucleic acid (DNA) technology in plant breeding offers an opportunity to enhance and accelerate genetic gain. Molecular markers, which are DNA or gene sequences with known locations on chromosomes are used as a tool for identification and genetic analysis. DNA extraction is the starting point and a critical step for genetic analysis. DNA can be extracted from diverse sources, including seeds and leaves of plants. Proper plant collection and storage conditions are essential for successfully extracting DNA of high quality and quantity from plants.

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Creator Abiodun Olutegbe
Creator Affiliation International Institute of Tropical Agriculture (IITA)
Creator email A.Olutegbe@cgiar.org
Creator ID Type ORCID
Creator ID 0009-0009-4223-0676
Subject Vocab (AGROVOC/GACS/CAB)
Subject(s)
Publisher International Institute of Tropical Agriculture (IITA)
Contributor Person 1 Adaobi Nnadi
Contributor Person 1 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 1 email L.Nnadi@cgiar.org
Contributor Person 2 Adenike Ige
Contributor Person 2 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 2 email A.Ige@cgiar.org
Contributor Person 3 Kehinde Nafiu
Contributor Person 3 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 3 email K.Nafiu@cgiar.org
Contributor Person 4 Bukola Ogungbesan
Contributor Person 4 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 4 email B.Ogungbesan@cgiar.org
Contributor Person 5 Ismail Kayondo
Contributor Person 5 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 5 email S.Kayondo@cgiar.org
Contributor Person 6 Mbanjo-Edwige Gaby-Nkouaya
Contributor Person 6 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 6 email E.Mbanjo@cgiar.org
Contributor Person 7 Ismail Rabbi
Contributor Person 7 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 7 email I.Rabbi@cgiar.org
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Contributor Project Lead Organisation Center International Institute of Tropical Agriculture (IITA)
Contributor Project Lead Center
Contributor Initiative/CRP Non-Initiative/CRP
Contributor Partner
Contributor Donor Not Applicable
Contributor Project Not Applicable
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Open-Access status Limited Access
Production Date 2024-06-10
Embargo End Date
Content Type Training Material
File Format pdf
Identifier Type DOI
Identifier https://doi.org/10.25502/jtd3-t271/d
Identifier Citation
Source
Language English
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Contact Abiodun Olutegbe; International Institute of Tropical Agriculture (IITA)
Contact Email A.Olutegbe@cgiar.org
Restriction CC-BY 4.0
Email Permission None
Rights CC-BY 4.0