1. Home
  2. Organizations
  3. International Institute of ...
  4. Standard Operating ...

Standard Operating Procedures for Genotyping in Yam Breeding Program

Yam (Dioscorea species) leaf samples contain high levels of polysaccharides and polyphenolic compounds. The extraction of DNA from leaves is usually problematic. Therefore, there is a need to extract good quality DNA to perform downstream DNA analysis; the cetyltrimethylammonium bromide (CTAB) DNA. Yam breeding optimizes DNA extraction from both leaves and tubers using a modified protocol for high-quality DNA.

Citation

Data and Resources

Additional Info

Field Value
Creator Amele Asrat
Creator Affiliation International Institute of Tropical Agriculture (IITA)
Creator email A.Amele@cgiar.org
Creator ID Type ORCID
Creator ID 0000-0002-4859-0631
Subject Vocab (AGROVOC/GACS/CAB)
Subject(s)
Publisher International Institute of Tropical Agriculture (IITA)
Contributor Person 1 Agre Paterne
Contributor Person 1 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 1 email P.Agre@cgiar.org
Contributor Person 2 Salaudeen Nurudeen
Contributor Person 2 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 2 email N.salaudeen@cgiar.org
Contributor Person 3 Alex Edemodu
Contributor Person 3 Affiliation International Institute of Tropical Agriculture (IITA)
Contributor Person 3 email A.edemodu@cgiar.org
Contributor Person 4
Contributor Person 4 Affiliation
Contributor Person 4 email
Contributor Person 5
Contributor Person 5 Affiliation
Contributor Person 5 email
Contributor Person 6
Contributor Person 6 Affiliation
Contributor Person 6 email
Contributor Person 7
Contributor Person 7 Affiliation
Contributor Person 7 email
Contributor Person 8
Contributor Person 8 Affiliation
Contributor Person 8 email
Contributor Person 9
Contributor Person 9 Affiliation
Contributor Person 9 email
Contributor Person 10
Contributor Person 10 Affiliation
Contributor Person 10 email
Contributor Person 11
Contributor Person 11 Affiliation
Contributor Person 11 email
Contributor Person 12
Contributor Person 12 Affiliation
Contributor Person 12 email
Contributor Person 13
Contributor Person 13 Affiliation
Contributor Person 13 email
Contributor Person 14
Contributor Person 14 Affiliation
Contributor Person 14 email
Contributor Person 15
Contributor Person 15 Affiliation
Contributor Person 15 email
Contributor Person 16
Contributor Person 16 Affiliation
Contributor Person 16 email
Contributor Person 17
Contributor Person 17 Affiliation
Contributor Person 17 email
Contributor Person 18
Contributor Person 18 Affiliation
Contributor Person 18 email
Contributor Person 19
Contributor Person 19 Affiliation
Contributor Person 19 email
Contributor Project Lead Organisation Center International Institute of Tropical Agriculture (IITA)
Contributor Project Lead Center
Contributor Initiative/CRP Non-Initiative/CRP
Contributor Partner
Contributor Donor Not Applicable
Contributor Project Not Applicable
Project ID
Contributor Affiliation
Open-Access status Open Access
Production Date 2022-06-15
Embargo End Date
Content Type Training Material
File Format pdf
Identifier Type DOI
Identifier https://doi.org/10.25502/hr6d-d521/d
Identifier Citation
Source
Language English
Relation
Agroecological Zone
Coverage Region
Coverage country
Coverage Admin Unit
Coverage Y (Latitude)
Coverage X (Longitude)
Coverage Start Date
Coverage End Date
Contact Amele Asrat; International Institute of Tropical Agriculture (IITA)
Contact Email A.Amele@cgiar.org
Restriction CC-BY 4.0
Email Permission None
Rights CC-BY 4.0