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STANDARD OPERATING PROCEDURE (SOP) FOR HARVESTING AND HARVEST EVALUATION IN C...
In cassava breeding, harvesting is carried out to determine the root yield and assess other important traits. It marks the end of the fieldwork for an experimental cassava... -
STANDARD OPERATING PROCEDURES FOR PHENOTYPING DATA MANAGEMENT IN IITA CASSAVA...
Data management involves data collection, curation, analysis, and storage to ensure accessibility to quality data. The goal of data management is to provide breeders with... -
STANDARD OPERATING PROCEDURE (SOP) FOR LEAF SAMPLE COLLECTION FOR GENOTYPING
Application of deoxyribonucleic acid (DNA) technology in plant breeding offers an opportunity to enhance and accelerate genetic gain. Molecular markers, which are DNA or gene... -
Standard Operating Procedure (SOP) FOR DNA EXTRACTION
Deoxyribonucleic acid (DNA) extraction is an important process in molecular plant breeding. DNA extraction involves isolating DNA from the nucleus of a cell. Once purified,... -
Standard Operating Procedure (SOP) for In vitro high throughput (HTP) phenot...
Parasitic nematodes (PPNs) mainly Radopholus spp. also known as burrowing nematodes are a major biotic threat to banana production in most African banana-growing regions... -
STANDARD OPERATING PROCEDURE (SOP) FOR HYBRIDIZATION AND CROSSING BLOCK MANAG...
Genetic variation is the basis of selection in plant breeding. Hybridization is the process of making controlled crosses between selected genotypes to provide genetic variation... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP...
Genotyping involves studying an organism’s genetic makeup based on its DNA. Banana leaves are commonly used for DNA extraction due to their abundance, accessibility, and the... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP...
Genotyping involves studying an organism’s genetic makeup based on its DNA. Banana leaves are commonly used for DNA extraction due to their abundance, accessibility, and the... -
Standard Operating Procedure (SOP) for Banana Embryo Culture
Banana/plantain breeding depends on successful controlled hybridization (or open pollinations) resulting in the formation of fertile seeds. However, most banana seeds fail to... -
Standard Operating Procedure (SOP) for banana tissue culture media preparation
Plant tissue culture involves growing plant cells, tissues, or organs on a sterile growth medium in vitro under controlled conditions of temperature, light, and humidity. An... -
Standard Operating Procedure (SOP) Site Selection & Field Layout
Site selection and field plot layout are important components of experimentations and require adequate attention. Poor site selections and field layouts are major sources of... -
Guidelines to prepare for digital data collection
This document lists the different tasks to be performed by the use case team before and during the enumerators’ training to ensure digital data collection in the field is smooth. -
Standard Operating Procedure for Yam Data Collection
Accurate data collection is essential for precise decisions in yam breeding activities. It is relevant for reliable choices in selecting and advancing desirable genotypes. -
Standard Operating Procedure for Yam Data Management
The yam breeding generates thousands of genotypic and phenotypic data every year from genetic studies and breeding trials. There is a need for data centralization, curation,... -
Standard Operating Procedures for Genotyping in Yam Breeding Program
Yam (Dioscorea species) leaf samples contain high levels of polysaccharides and polyphenolic compounds. The extraction of DNA from leaves is usually problematic. Therefore,... -
Standard Operating Procedure for Harvesting and Post-Harvest Tuber Management...
The tuber is the food organ and the essential part of the yam plant. Evaluations and selection are premised on this economic part, just like in other root ad tuber crops. The... -
Standard Operating Procedure for Yam Trial Management
Yam trial management involves all the activities implemented throughout the life cycle of a yam trial/experiment, either in the field, screenhouse and laboratory, following a... -
Standard Operating Procedure for Regional Varietal Trial
The yam breeding program executes regional varietal trials (RVTs) in collaboration with its national partners in different countries. The objective of the trial is to... -
Standard Operating Procedure for Field Trial Labelling and Barcoding
Barcode labels are used for trials plot, genotypes identification and tracking purposes. For the labelling of a trial, this includes stages of advancement, the location of the... -
Standard Operating Procedure for Yam Crossing
Hybridization in yam is a process of transferring of male pollens to female flowers to generate viable seeds with desired genetic recombination of the parents. Hybridization...
