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Standard Operating Procedure (SOP) for Phenotyping Banana for Resistance to t...
Plant-parasitic nematodes (PPNs) such as Pratylenchus spp., Helicotylenchus spp., and Radopholus spp. amongst others are a major constraint to sustainable bananas (Musa spp.)... -
Standard Operating Procedure (SOP) for Pollen Tube Growth and Monitoring (PTGM)
This procedure allows for assessing pollen viability, compatibility, and fertilization efficiency, essential for improving hybridization success and enhancing hybrid recovery... -
Standard Operating Procedure (SOP) for Phenotypic Field data collection and m...
This SOP provides detailed procedures for data collection, analysis, and statistical design for hybrid evaluations in the banana breeding program. It ensures data quality,... -
Standard Operating Procedure (SOP) for Collecting, Preparing, Analyzing, and ...
This SOP provides detailed procedures for collecting, preparing, quantifying, analyzing, and assessing banana pollen grains in a consistent and reproducible manner that ensures... -
Standard Operating Procedure (SOP) for land preparation and planting Banana a...
This Standard Operating Procedure (SOP) provides a comprehensive guide to best practices for land preparation and planting of banana and plantain fields. It aligns with... -
Standard Operating Procedure (SOP) for Harvesting Banana and Plantain
This Standard Operating Procedure (SOP) provides comprehensive, detailed guidelines for banana harvest activities that include the fruit handling, the data collection and data... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP)...
Unlike seed crops, clonally propagated crops are difficult and expensive to conserve. With the rapidly evolving genotyping platforms, it is recommended to conserve leaf samples... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP...
Genotyping involves studying an organism’s genetic makeup based on its DNA. Banana leaves are commonly used for DNA extraction due to their abundance, accessibility, and the... -
IITA MUSA IMPROVEMENT – MOLECULAR BREEDING STANDARD OPERATING PROCEDURE (SOP...
Genotyping involves studying an organism’s genetic makeup based on its DNA. Banana leaves are commonly used for DNA extraction due to their abundance, accessibility, and the... -
Standard Operating Procedure (SOP) for Banana Embryo Culture
Banana/plantain breeding depends on successful controlled hybridization (or open pollinations) resulting in the formation of fertile seeds. However, most banana seeds fail to... -
Standard Operating Procedure (SOP) for banana tissue culture media preparation
Plant tissue culture involves growing plant cells, tissues, or organs on a sterile growth medium in vitro under controlled conditions of temperature, light, and humidity. An... -
Standard Operating Procedure (SOP) for digital imaging and quantification of ...
The quantification of weevil damage on banana corms is an important task when assessing resistance. In the process of analysing hundreds of samples for weevil damage, there is... -
Standard Operating Procedure (SOP) for In vitro high throughput (HTP) phenot...
Parasitic nematodes (PPNs) mainly Radopholus spp. also known as burrowing nematodes are a major biotic threat to banana production in most African banana-growing regions... -
Standard Operating Procedure (SOP) for Ploidy Analysis in Banana
Banana is a natural multi-ploid crop. Wild bananas are seeded diploids (2x), while banana cultivars are seedless, mostly triploid (3x) and sometimes diploid. Because of the... -
Standard Operating Procedure (SOP) for Phenotyping and Data Management of Ea...
The hybrids developed from the different cross combinations undergo a series of trials starting with the early evaluation trial (EET), in which only two replications per... -
Standard Operating Procedure (SOP) for Field Establishment of Banana crosses
The hybrids developed from the different cross combinations undergo a series of trials starting with the early evaluation trial (EET), in which only two replications per... -
Standard Operating Procedure (SOP) for Banana Hybridization
The purpose of this document is to describe the SOPs for banana breeding activities and data management. These activities include crossing, evaluation trial establishment,... -
Standard Operating Procedure (SOP) for Banana Bacterial Wilt Resistance Scree...
Banana is greatly affected by a bacterial pathogen Xanthomonas vasicola pv. musacearum (Xvm), that causes banana bacterial wilt.1 It is reported to be the most devastating... -
Standard Operating Procedure (SOP) for screening for resistance to Black Siga...
Banana, and especially the cooking type known as East Africa highland banana, is a vital staple food and income crop for over 80 million people in East Africa. However,... -
Standard Operating Procedure (SOP) for Screening Banana resistance against Fu...
Banana (Musa spp.) is an important staple and source of income for many people in developing countries. Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc) race 1,...